Technology revolutionizes dioxin screens to 1ppt with 24- to 48-hour turnaround

Go to Procept Information

CHICAGO – (August 11, 2005) – Eichrom Technologies, Inc., today announced it will unveil the fastest, most cost-efficient method of low detection limit screening for dioxins at Dioxin 2005, August 21-26, in Toronto. The test for the carcinogenic compounds in the environment and food supply linked to petroleum-derived herbicides and other products will be marketed to analytical laboratories as the Procept Rapid Dioxin Assay.

“Procept allows chemistry labs to screen for dioxins down to 1 part per trillion (ppt) in as little as 24 hours,” said Michael Fern, Vice President, Sales and Marketing, Eichrom Technologies, Inc. “Before Procept, dioxin analysis meant long turnaround times, high detection limits or expensive equipment, such as gas chromatography-mass spectrometry (GC-MS) or tissue culture infrastructure. Procept eliminates all of these issues.”

Eichrom will package Procept as a turn-key solution for analytical chemistry laboratories seeking a user-friendly way to screen for dioxins. Procept:

• Reduces cost and time compared to traditional GC-MS tests.
• Improves detection limits compared to immunoassay tests.
• Eliminates need for live cells used by reporter gene assays.
• Comes with training, methodology, and technical support needed to perform dioxin screens.
• Is available in kits for 24, 48 or 96 tests.

Procept has been successfully evaluated by the Japanese Ministry of the Environment for the analysis of incinerator ash and stack gas samples, and is expected to be included in the US Environmental Protection Agency’s SW-846 method manual in early 2006. Procept can be used now for non-EPA-required tests.

“Procept is revolutionary technology,” said Fern. “For the first time, chemists will be able to generate in-house up to 75 analytical results per day at 1 ppt detection limit. Subcontracting to other labs, lengthy turn-around and high costs are gone with Procept.”

How Procept Works
Procept is based on the aryl hydrocarbon receptor (Ah Receptor) interaction with dioxin and dioxin-like compounds, the biological basis for the toxicity of these molecules. In the cell, dioxin is conjugated by the Ah Receptor and ARNT, a nuclear transcription factor which transports the dioxin into the cell nucleus. In the nucleus the Ah Receptor connects the dioxin molecule with DNA, resulting in the toxicity to the living organism. The Procept dioxin assay utilizes the Ah Receptor molecule to detect the presence of dioxin in a sample. A special DNA sequence is added to the sample mix. If dioxin is present, the DNA will be bound to the Ah Receptor-Dioxin conjugate. The DNA fragment is then amplified using a technology called PCR (short for Polymerase Chain Reaction.) As the DNA is doubled over and over again, the PCR instrument measures how much is present. The amount of DNA correlates directly to the amount of dioxin in the sample. The technique is very sensitive and has a linear response.

The technology behind Procept was developed by Hybrizyme Corporation and previously marketed worldwide under the name, AhRC PCR. Eichrom is now the exclusive licensee of this technology in the US and Europe.

Visit Eichrom at Dioxin 2005 – Exhibit 211
Eichrom Technologies will exhibit Procept from August 21-26 at Dioxin 2005, the 25th International Symposium on Halogenated Environmental Organic Pollutants and POPs at the Fairmount Royal York Hotel in downtown Toronto.

About Eichrom
Eichrom Technologies, Inc., is a worldwide provider of products and services in the field of analytical chemistry. Eichrom’s state-of-the-art chemical separation products, high-performance laboratory filters and analytical test kits bring value to Eichrom customers through increased speed, safety and reproducibility. Eichrom methods are simpler to use, meeting the objectives of production managers, controllers and analysts alike. Eichrom Technologies has been registered by SGS to the ISO 9000 series for quality: ISO 9001:2000.